Rafatunisa Nahri


Annona squamosa belongs to the family Annonaceae, commonly known as custard apple. A comparative antibacterial activity of methanolic leaf and seed cotyledon extracts of Annona squamosa were evaluated against three bacterial strains namely Bacillus subtilis, Escherichia coli, staphylococcus aureus using agar well diffusion method. Maximum inhibition was found with 40 mg/ml concentration of methanolic leaf and seed cotyledon extracts against all the tested organisms under investigation. The minimum inhibitory concentrations were determined by disk diffusion method. To find the compound responsible for antibacterial activity thin layer chromatography was done.The FTIR analysis was performed represented the presence of various functional groups. The phytochemical analysis was performed. The study suggests that the leaf and seed cotyledon extracts of Annona squamosa are promising the development of phytomedicine for antimicrobial properties.


Annona squamosa, Bacterial strains, Discdiffusion, Methanolic extract.

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